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Slowdown of surface diffusion during early stages of bacterial colonization

T. Vourc'h and H. Peerhossaini*

J. Léopoldès†

A. Méjean

F. Chauvat and C. Cassier-Chauvat

  • Laboratoire AstroParticules et Cosmologie, CNRS, Université Paris–Diderot, Université Sorbonne Paris Cité, 5 Rue Thomas Mann, 75013 Paris, France

  • ESPCI Paris, PSL Research University, CNRS, Institut Langevin, 1 Rue Jussieu, 75005 Paris, France and Université Paris–Est Marne-la-Vallée, 5 Boulevard Descartes, Champs sur Marne, Marne-la-Vallée Cedex 2, France

  • Laboratoire Interdisciplinaire des Énergies de Demain, CNRS, Université Paris–Diderot, Université Sorbonne Paris Cité, 5 Rue Thomas Mann, 75013 Paris, France

  • Laboratory of Biology and Biotechnology of Cyanobacteria. Institute for Integrative Biology of the Cell, CEA, CNRS, Université Paris–Sud, Université Paris–Saclay, 91198, Gif-sur-Yvette Cedex, France

  • *hassan.peerhossaini@univ-paris-diderot.fr
  • †julien.leopoldes@espci.fr
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Phys. Rev. E 97, 032407 – Published 14 March, 2018

DOI: https://doi.org/10.1103/PhysRevE.97.032407

Abstract

We study the surface diffusion of the model cyanobacterium Synechocystis sp. PCC6803 during the incipient stages of cell contact with a glass surface in the dilute regime. We observe a twitching motility with alternating immobile tumble and mobile run periods, resulting in a normal diffusion described by a continuous-time random walk with a coefficient of diffusion D. Surprisingly, D is found to decrease with time down to a plateau. This is observed only when the cyanobacterial cells are able to produce released extracellular polysaccharides, as shown by a comparative study between the wild-type strain and various polysaccharides-depleted mutants. The analysis of the trajectories taken by the bacterial cells shows that the temporal characteristics of their intermittent motion depend on the instantaneous fraction of visited sites during diffusion. This describes quantitatively the time dependence of D, related to the progressive surface coverage by the polysaccharides. The observed slowdown of the surface diffusion may constitute a basic precursor mechanism for microcolony formation and provides clues for controlling biofilm formation.

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Phys. Rev. E 97, 032407– Published 14 March, 2018
  • Received 10 November 2017
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