Skip to Main Content

Access Provided by Xinjiang University

Physical Review E

Access by Xinjiang University

Assessment of radiation damage in single-shot coherent diffraction of DNA molecules by an extreme-ultraviolet free-electron laser

Jaehyun Park¹, Daewoong Nam^1,2, Yoshiki Kohmura¹, Mitsuru Nagasono¹, Yongmoon Jeon², Jong-Bong Lee², Tetsuya Ishikawa¹, and Changyong Song^1,*

¹RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan
²Department of Physics, Pohang University of Science and Technology (POSTECH), Pohang 790-784, Korea

^*Author to whom correspondence should be addressed: cysong@spring8.or.jp

Phys. Rev. E 86, 042901 – Published 25 October, 2012

DOI: https://doi.org/10.1103/PhysRevE.86.042901

Abstract

We have investigated the progress of structural distortions in DNA molecules by single-shot coherent diffraction using extreme-ultraviolet radiation from a free-electron laser. A speckle pattern of DNA molecules was successfully acquired using photons in a single pulse with a 100 fs pulse width. The radiation damage was assessed by a cross correlation, revealing that the first exposure has significantly deformed most of the original structures. Molecules were not completely destroyed by the first single-shot exposure and underwent subsequent distortions through continued exposure, until eventually deforming into a radiation-hard structure.

Article Text

References (23)

D. Sayre, Acta Crystallogr. 5, 843 (1952).
J. Miao et al., Nature (London) 400, 342 (1999).
H. N. Chapman and K. A. Nugent, Nat. Photonics 4, 833 (2010).
J. Miao, R. L. Sandberg, and C. Song, IEEE J. Sel. Top. Quantum Electron. 18, 399 (2011).
R. Neutze et al., Nature (London) 406, 752 (2000).
J. Miao, K. O. Hodgson, and D. Sayre, Proc. Natl. Acad. Sci. USA 98, 6641 (2001).
H. Jiang et al., Proc. Natl. Acad. Sci. USA 107, 11234 (2010).
C. Song, H. Jiang, A. Mancuso, B. Amirbekian, L. Peng, R. Sun, S. S. Shah, Z. H. Zou, T. Ishikawa, and J. Miao, Phys. Rev. Lett. 101, 158101 (2008).
Y. Nishino, Y. Takahashi, N. Imamoto, T. Ishikawa, and K. Maeshima, Phys. Rev. Lett. 102, 018101 (2009).
R. Fung et al., Nat. Phys. 5, 64 (2009).
W. Ackermann, Nat. Photonics 1, 336 (2007).
P. Emma et al., Nat. Photonics 4, 641 (2010).
B. W. J. McNeil and N. R. Thompson, Nat. Photonics 4, 814 (2010).
H. N. Chapman et al., Nat. Phys. 2, 839 (2006).
H. N. Chapman et al., Nature (London) 470, 73 (2011).
M. M. Seibert et al., Nature (London) 470, 78 (2011).
H. M. Quiney and K. A. Nugent, Nat. Phys. 7, 142 (2011).
S. P. Hau-Riege et al., Phys. Rev. Lett. 98, 145502 (2007).
A. R. Khorsand et al., Opt. Express 18, 700 (2010).
T. Shintake et al., Nat. Photonics 2, 555 (2008).
H. Ohashi et al., Nucl. Instrum. Methods Phys. Res., Sect. A 649, 163 (2010).
C. Bustamante et al., Science 265, 1599 (1994).
H. N. Chapman, Nature (London) 448, 676 (2007).