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Probing single DNA mobility with fluorescence correlation microscopy

Svetlana A. Tatarkova1,2,* and David A. Berk2

  • 1Department of Physics, University of Durham, Durham DH1 3LE, United Kingdom
  • 2School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Oxford Road, Manchester M13 9PL, United Kingdom

  • *Corresponding author. Electronic address: s.a.tatarkova@durham.ac.uk
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Phys. Rev. E 71, 041913 – Published 27 April, 2005

DOI: https://doi.org/10.1103/PhysRevE.71.041913

Abstract

Fluorescence correlation spectroscopy combined with microscopy (FCSM) is used to study the mobility of DNA fragments in aqueous solution and tissue models on the single molecule level. The effective hydrodynamic radius was measured for various lengths of ds-DNA chains and obeyed the theoretically inveterate [DNAlength]0.5 relationship. Hindered diffusion of ds-DNA through the gel matrix of various densities is thought of as an extension of Kramer’s problem for a flexible polymer chain. With increasing DNA length the average barrier crossing time rises as [DNAlength]2 and this agrees with theory predictions for polymer molecules surmounting an entropic barrier.

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Phys. Rev. E 71, 041913– Published 27 April, 2005
  • Received 20 August 2003

© 2005 American Physical Society

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