- Physical Review Journals
All JournalsPhysics Magazine
Access Provided by Xinjiang University

Physical Review E

Export citation

Export citation

Choose format for download:

Download Citation
  • Access by Xinjiang University

Miscibility gap in fluid dimyristoylphosphatidylcholine:cholesterol as “seen” by x rays

Frank Richter1,2,*, Gert Rapp3,†, and Leonard Finegold4,‡

  • 1European Molecular Biology Laboratory at DESY, Notkestraße 85, D-22603 Hamburg, Germany
  • 2Department of Physics, E22 Biophysics, TU Munich, D-85748 Garching, Germany
  • 3MPI for Colloid and Interface Sciences, c/o HASYLAB, DESY, Notkestraße 85, D-22603 Hamburg, Germany
  • 4Department of Physics, Drexel University, Philadelphia, Pennsylvania 19104

  • *Electronic address: efrichter@web.de
  • †Electronic address: gert.rapp@desy.de
  • ‡Electronic address: L@drexel.edu
PDF

Phys. Rev. E 63, 051914 – Published 26 April, 2001

DOI: https://doi.org/10.1103/PhysRevE.63.051914

Abstract

A binary mixture of dimyristoylphosphatidylcholine (DMPC) and cholesterol displays a fluid miscibility gap under excess water conditions. Effects due to the imperfect miscibility of the two amphiphiles are studied near to and far from thermodynamic equilibrium by time-resolved small angle x-ray diffraction. The experiment discloses that this mixture phase separates when leaving the miscibility gap upon heating, a transition that is not included in current phase diagrams. This transition appears to be reversible and shows a temperature hysteresis of only a few degrees. We suggest a model in which the transition is driven with increasing temperature by a movement of the cholesterol away from the hydrophilic-hydrophobic interface toward the hydrophobic core of the bilayer.

View figure in article

References (46)

  1. E. Sackmann, in Structure and Dynamics of Membranes, edited by R. Lipowsky and E. Sackmann (North-Holland, Amsterdam, 1995).
  2. Cholesterol in Membrane Models, edited by L. Finegold (CRC, Boca Raton, FL, 1993).
  3. J. H. Ipsen, O. G. Mouritsen, and M. J. Zuckermann, Biophys. J. 56, 661 (1989).
  4. R. N. McElhaney and T. P. W. McMullen, Biochim. Biophys. Acta 1234, 90 (1995).
  5. R. Bittman, Subcellular Chemistry (Plenum, New York, 1997).
  6. E. Strauss, Science 280, 1528 (1998).
  7. T. M. Bayerl and E. Sackmann, in Cholesterol in Membrane Models (Ref. [2]).
  8. P. F. F. Almeida, W. L. C. Vaz, and T. E. Thomson, Biochemistry 31, 6739 (1992).
  9. F. Richter, L. Finegold, and G. Rapp, Phys. Rev. E 59, 3483 (1999).
  10. P. A. Reis and H. M. McConnell, Proc. Natl. Acad. Sci. USA 86, 6445 (1989); C. L. Hirshfeld and M. Seul, J. Phys. (France) 51, 1537 (1990).
  11. E. Sackmann, Can. J. Phys. 68, 991 (1990).
  12. M. P. Marsan et al., Biophys. J. 76, 351 (1999).
  13. O. G. Mouritsen, Chem. Phys. Lipids 57, 179 (1991).
  14. M. J. Zuckermann, J. H. Ipsen, and O. G. Mouritsen, in Cholesterol in Membrane Models (Ref. [2]).
  15. J. R. Silvius, D. del Giudice, and M. Lafleur, Biochemistry 35, 15 198 (1996).
  16. L. Finegold and M. A. Singer, in Cholesterol in Membrane Models (Ref. [2]).
  17. G. Rapp, A. Gabriel, M. Dosière, and M. H. J. Koch, Nucl. Instrum. Methods Phys. Res. A 357, 178 (1995).
  18. G. Rapp and R. S. Goody, J. Appl. Crystallogr. 24, 857 (1991).
  19. T. C. Huang, H. Toraya, T. N. Blanton, and Y. Wu, J. Appl. Crystallogr. 26, 810 (1993).
  20. A. Guinier, Principles of X-ray Diffraction (Freeman, San Francisco, 1963), Chap. 9.
  21. R. Zhang et al., Phys. Rev. Lett. 74, 2832 (1995).
  22. J. Lemmich et al., Phys. Rev. E 53, 5169 (1996).
  23. R. Zhang et al., Biophys. J. 70, 349 (1996).
  24. R. Zhang, R. M. Suter, and J. F. Nagle, Phys. Rev. E 50, 5047 (1994).
  25. D. Marsh, A. Watts, and P. F. Knowles, Biochemistry 15, 3570 (1976).
  26. S. Subramaniam and H. M. McConnell, J. Phys. Chem. 91, 1715 (1991).
  27. J. Lemmich et al., Eur. Biophys. J. 25, 293 (1997).
  28. A. M. Petrascu, M. H. J. Koch, and A. Gabriel, J. Macromol. Sci., Phys. 37, 463 (1998).
  29. M. Rappolt et al., Eur. Biophys. J. 29, 125 (2000).
  30. J. S. Langer, Ann. Phys. (N.Y.) 65, 53 (1971).
  31. I. M. Lifshitz and V. Slyozov, J. Phys. Chem. Solids 19, 35 (1961).
  32. L. K. Nielsen et al., J. Phys.: Condens. Matter 12, A309 (2000).
  33. S. Halstenberg et al., Biophys. J. 75, 264 (1998).
  34. C. Gliss et al., Biophys. J. 77, 331 (1999).
  35. M. Pasenkiewicz-Gierula et al., Biophys. J. 78, 1376 (2000).
  36. H. S. Shieh, L. G. Hoard, and C. E. Nordman, Nature (London) 267, 287 (1977); Acta Crystallogr., Sect. B: Struct. Crystallogr. Cryst. Chem. 37, 1538 (1981).
  37. W. Knoll, G. Schmidt, and E. Sackmann, J. Chem. Phys. 79, 3439 (1983).
  38. J. Huang, J. T. Buboltz, and G. W. Feigenson, Biochim. Biophys. Acta 1417, 89 (1999); J. Huang and G. W. Feigenson, Biophys. J. 76, 2142 (1999).
  39. P. R. ten Wolde and D. Frenkel, Science 277, 1975 (1997).
  40. D. J. Adams and I. R. McDonald, J. Chem. Phys. 63, 1900 (1975); ibid.R. P. Sear, 111, 4800 (1999).
  41. C. J. Dekker et al., Chem. Phys. Lipids 33, 93 (1983).
  42. R. Lipowsky, J. Phys. II 2, 1825 (1992).
  43. R. Koynova, B. Tenchov, and G. Rapp, Chem. Phys. Lipids 88, 45 (1997).
  44. G. L. Kirk and S. M. Gruner,, J. Phys. 46, 761 (1985); D. P. Siegel, J. P. Banschbach, and P. L. Yeagle, Biochemistry 28, 5010 (1989).
  45. Z. Chen and R. P. Rand, Biophys. J. 74, 944 (1998).
  46. T. Söderlund, J. Y. A. Lehtonen, and P. K. J. Kinnunen, Mol. Pharmacol. 55, 32 (1998).

Outline

Information

Phys. Rev. E 63, 051914– Published 26 April, 2001
  • Received 21 May 2000

© 2001 American Physical Society

Sign In to Your Journals Account

Filter

Filter

Article Lookup

Enter a citation