Observation of a Rectangular Columnar Phase in Condensed Lamellar Cationic Lipid-DNA Complexes

F. Artzner; R. Zantl; G. Rapp; J. O. Rädler

doi:10.1103/PhysRevLett.81.5015

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Observation of a Rectangular Columnar Phase in Condensed Lamellar Cationic Lipid-DNA Complexes

F. Artzner^1,*, R. Zantl¹, G. Rapp², and J. O. Rädler^1,†

¹Lehrstuhl für Biophysik, E22, Technische Universität München, James-Franck-Strasse 1, D-85748 Garching, Germany
²European Molecular Biology Laboratory, EMBL c/o DESY, Notkestrasse 85, D-22603 Hamburg, Germany

^*Permanent address: Equipe Physico-chimie des systèmes Polyphasés, CNRS URA 1218, 5, rue J. B. Clément F-92296 Châtenay-Malabry, France.
^†Email address: raedler@physik.tu-muenchen.de

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Phys. Rev. Lett. 81, 5015 – Published 30 November, 1998

DOI: https://doi.org/10.1103/PhysRevLett.81.5015

Abstract

We report on a synchrotron x-ray diffraction study of saturated cationic lipid complexed with DNA. In the lipid gel phase $L_{β^{'}}^{c}$ the complexes exhibit Bragg reflections due to lamellar lipid bilayer stacking and three nonintegral peaks in agreement with an intercalated centered rectangular columnar superlattice of DNA. The diffuse broadening of the DNA peaks is caused by in-plane translational disorder of the DNA strands and is approximated by Lorentzians with positional correlation lengths of $ξ_{y} \approx 250 Å$ out of plane and $ξ_{x} > 2000 Å$ in plane. We interpret our results in terms of a system of interacting two-dimensional smectic layers.

References (13)

V. Luzzati, in Biological Membranes, D. Chapman (Academic Press, London, 1967); F. Livolant, A. M. Levelut, J. Doucet, and J. P. Benoit, Nature (London) 339, 724-726 (1989); H. H. Strey, V. A. Parsegian, and R. Podgornik, Phys. Rev. Lett. 78, 895–898 (1997).
J. O. Rädler, I. Koltover, T. Salditt, and C. R. Safinya, Science 275, 810 (1997).
T. Salditt, I. Koltover, J. O. Rädler, and C. Safinya, Phys. Rev. Lett. 79, 2582 (1997).
C. S. O'Hern and T. C. Lubensky, Phys. Rev. Lett. 80, 4345 (1998).
L. Golubovic̀ and M. Golubovic̀, Phys. Rev. Lett. 80, 4341 (1998).
R. Zantl, F. Artzner, G. Rapp, and J. O. Rädler, Eur. Phys. Lett. (to be published).
I. Koltover, T. Salditt, J. O. Rädler, and C. Safinya, Science 281, 78 (1998).
F. Artzner, R. Zantl, and J. O. Rädler (unpublished).
A. J. Leadbetter, J. C. Frost, and M. A. Mazid, J. Phys. Lett. (Paris) 40, 325 (1979).
The analysis is analogous to the treatment of planar disorder in layered crystals as, for example, graphite or various clays; see, e.g., A. Guinier, X-Ray Diffraction in Crystals, Imperfect Crystals, and Amorphous Bodies (Dover Publication, New York, 1994), p. 219–237.
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P. G. De Gennes and J. Prost, The Physics of Liquid Crystals (Clarendon Press, Oxford, 1993).
B. J. Battersby et al., BBA 1372(2), 379 (1998).

[c1] V. Luzzati, in Biological Membranes, D. Chapman (Academic Press, London, 1967); F. Livolant, A. M. Levelut, J. Doucet, and J. P. Benoit, Nature (London) 339, 724-726 (1989); H. H. Strey, V. A. Parsegian, and R. Podgornik, Phys. Rev. Lett. 78, 895–898 (1997).

[c2] J. O. Rädler, I. Koltover, T. Salditt, and C. R. Safinya, Science 275, 810 (1997).

[c3] T. Salditt, I. Koltover, J. O. Rädler, and C. Safinya, Phys. Rev. Lett. 79, 2582 (1997).

[c4] C. S. O'Hern and T. C. Lubensky, Phys. Rev. Lett. 80, 4345 (1998).

[c5] L. Golubovic̀ and M. Golubovic̀, Phys. Rev. Lett. 80, 4341 (1998).

[c6] R. Zantl, F. Artzner, G. Rapp, and J. O. Rädler, Eur. Phys. Lett. (to be published).

[c7] I. Koltover, T. Salditt, J. O. Rädler, and C. Safinya, Science 281, 78 (1998).

[c8] F. Artzner, R. Zantl, and J. O. Rädler (unpublished).

[c9] A. J. Leadbetter, J. C. Frost, and M. A. Mazid, J. Phys. Lett. (Paris) 40, 325 (1979).

[c10] The analysis is analogous to the treatment of planar disorder in layered crystals as, for example, graphite or various clays; see, e.g., A. Guinier, X-Ray Diffraction in Crystals, Imperfect Crystals, and Amorphous Bodies (Dover Publication, New York, 1994), p. 219–237.

[c11] D. Harries, S. May, W. M. Gelbart, and A. Ben-Shaul, Biophys. J. 75, 159 (1998); R. Bruinsma, Eur. Phys. J. B 4, 75 (1998).

[c12] P. G. De Gennes and J. Prost, The Physics of Liquid Crystals (Clarendon Press, Oxford, 1993).

[c13] B. J. Battersby et al., BBA 1372(2), 379 (1998).

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Observation of a Rectangular Columnar Phase in Condensed Lamellar Cationic Lipid-DNA Complexes

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